Activity of site-specific endonucleases on complexes of plasmid DNA with multiwalled carbon nanotubes

Abstract

Study of nuclease activity is important for understanding reasons of variability of deoxyribonucleic-acid (DNA) structure. Living cells utilize cutting and cleavage of DNA molecules by DNA-hydrolyzing enzymes: DNase, endonuclease, and other various nucleases to recognize and restrict genetic infection. Nuclease activity is proposed to use for revealing, for example, genes with single nucleotide polymorphisms through formation of a three-dimensional structure due to perfect hybridization of DNA with oligonucleotides and then subsequent cutting of the three-dimensional structure by nucleases.